FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13644160
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17200.016.3990271
GTATTAG28650.015.8482761
GTCCTAG34300.015.6445531
GTATTAA43500.014.7871691
GTATACG6200.014.4249521
GTCTTAG33100.014.1333011
GTGCTAG35200.013.9741721
GCTCTAG40750.013.5903611
GTATTAT42550.013.5004921
GTTCTAG42700.013.4530671
GTACTAA27200.013.4050871
GTATAAG30950.013.3368511
GTACATA39900.013.1039841
TATACTG42150.012.9759815
GTATATA56700.012.9219871
CTAGACT30350.012.6939974
GTCCTAA32300.012.6729331
GGTCTAA24750.012.5083481
GTCCTAT30400.012.3334811
GTGCTAA32950.012.2141481
Produced by FastQC (version 0.11.5)