FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13644160
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG31350.016.6855811
GTACTAG16900.015.883391
GTCCTAG36100.015.2527471
GTACTAA27350.014.5960711
GTTCTAG41900.014.4555271
GTCTTAG35800.014.4192791
GTATTAA46850.013.8096771
GTATATA58450.013.777381
GTATAAG28550.013.5004181
CCTATAC25050.013.4573963
GTTCTAA43800.013.2784651
GTATAGG22650.013.2186271
GCCCTAG33550.013.0270611
GGTCTAA26700.013.0180341
GTATTAT42800.012.9454581
GTACAGG59550.012.8871951
CTAGACT33650.012.778144
GTCCTAT29200.012.7284691
TGCACTG86050.012.7121475
GGTCTAG28400.012.6023141
Produced by FastQC (version 0.11.5)