FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8537187
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA58650.024.42284145
GTCCTAG36900.019.8797931
GTACTAG18800.019.8403631
GTACTAA29550.018.0924421
GTTCTAG39700.016.7551961
GTCTTAG33900.015.2206991
GTATTAA53950.014.74941351
GTGTAGG49800.014.73020651
GCTCTAG37900.014.5984591
GTATTAG32200.014.4797671
TCTATAC34100.014.402373
GTACAGG64600.014.2424731
CTGTACT71700.014.2197224
GTTCTAA47050.013.8734951
GTCTTAA42200.013.8475081
TATACTG47700.013.8150965
GTGTATA42850.013.6374521
GTGCTAG37850.013.4680341
CTATACT36250.013.3768234
CTAGACT29850.013.3291374
Produced by FastQC (version 0.11.5)