FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462509
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA60300.028.233906145
GTCCTAG33150.018.9066411
GTACTAG17800.018.477041
CTATACT33650.018.258184
GTGCTAG35550.016.757461
GTATTAG32900.015.6552051
GCTCTAG42000.015.51374151
GTATAGG24550.015.4189531
GTCTTAG32850.015.112321
GTTCTAG41900.014.9583581
TCTAGAC31050.014.3897273
CTAGGCC41950.014.0539694
CTAGACT29850.013.7216694
GGGGGGG423100.013.61019145
GGTCTAG27000.013.560161
GCCTTAG33700.013.4421741
GTACAGG64600.013.3523851
TAGACAG53000.013.3486985
CTACACT40450.013.347754
TATACTG45350.013.2740785
Produced by FastQC (version 0.11.5)