FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7142932
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG27100.017.91307673
GTACTAG9900.014.7485421
GTACTAA13900.014.4434911
ACGAGAC36900.013.94699273
GTCCTAA15200.013.928641
GTCCTAG14700.013.9057681
GTATTAG15250.013.8829731
GTCTTAA21350.013.6778061
GCGTTAA2951.260787E-613.6111551
TCCGAGC159000.013.33726773
GTCTTAG15950.013.2736871
GCGCTAG2253.334672E-412.9787171
GTATAGG11450.012.1144141
GTATTAA22150.012.0302161
GGTCTAG13400.011.9859431
GTATATA23750.011.8345411
GTTCTAG20700.011.8148651
GTTCTAA22350.011.759241
CACGAGA46950.011.66120673
CCGAGCC136400.011.55998373
Produced by FastQC (version 0.11.5)