FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7142932
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG14800.017.0238231
GTCCTAA14550.015.5596021
GACGAAT31200.015.20856673
GTACTAG8700.015.1096031
CGACGAA35100.014.76660673
GGTCTAA13050.013.7105651
GTGCTAG17850.013.7058621
TGACGCT155000.013.44633473
CTAGCGC2202.7589715E-413.272933
GTTCTAG19750.012.9419791
GTACTAA13400.012.8074541
CCGACGA43300.012.56023973
ACGAATA22550.012.3017473
GTATTAA24100.012.2726331
GTCTTAG15300.012.1716241
CTAGACT14400.012.1666824
CGAATAG17350.011.99153773
GCTCTAG17750.011.931621
GACGCTG135250.011.87448873
GTATTAG13550.011.8572271
Produced by FastQC (version 0.11.5)