FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6950960
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG3056.9776434E-915.5576521
GTGCTAG15650.015.3932461
GTACTAG8700.015.1037181
GTCCTAG15100.014.9870211
CGAGACG25300.014.8595273
GTCTTAG14700.014.4016171
ACGAGAC34200.014.087563573
CCGAGCC129350.013.65736873
TCCGAGC151350.013.36027473
GTATAGA17150.013.195571
GTCCTAA14250.012.8072441
CGATCGA2000.002030850612.77568510
CGAGCCC100750.012.57107773
GTTCTAA19800.012.3512291
GTCTAAG13700.012.25569251
GGTCTAG11800.012.0637731
GTCTATA13650.011.7657771
GCCTTAG15850.011.7446881
GCTCTAG18600.011.5781621
GTACAGG27850.011.5334191
Produced by FastQC (version 0.11.5)