FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7258929
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG27650.016.50083273
GTGCTAG18250.014.800651
GTCCTAG17850.013.4963891
TCCGAGC158100.013.41328773
CCGAGCC134300.013.39867673
GTCTTAG16650.013.1537311
GTATAGG11100.013.153731
GTTCTAG19000.013.0637321
GTTCTAA21200.012.39677051
GTCCTAA15900.012.39677051
GTATTAG14850.012.2901021
ACGAGAC37100.012.19940872
GGTCTAA13400.011.98561
GTACATG27750.011.5752831
GGTCTAG12300.011.5736781
GTGTAGG21550.011.5179081
GTACTAG8250.011.5035341
GGCTTAG17250.011.4265891
CTCCGAG194750.011.39507373
TCTATAC15850.011.2845623
Produced by FastQC (version 0.11.5)