FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7124100
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG27150.016.26683273
GTTCTAG19100.015.8642861
GTACTAG7850.013.951711
GTGCTAG17250.013.5446171
ACGAGAC36650.013.245473
CCGAGCC136500.013.022181573
GTCCTAG16150.012.6587651
TCCGAGC155400.012.63626473
GTATTAG12750.012.5984851
GTCTTAG15850.012.4377071
TCAGTAC20050.012.3807223
GTGCTAA19050.012.264811
GTACTAA13100.012.2618841
GCCCTAG14400.012.1689911
CGAGCCC104450.012.02091273
CTATACT16850.011.4822614
GTATTAA22950.011.4531691
CACGAGA46550.011.44776273
GTGTTAG16500.011.2839741
ACTATAC12950.011.2756533
Produced by FastQC (version 0.11.5)