FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783817
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG6250.020.350461
TCTAGAC5000.014.4383463
TATACTA5350.014.1363425
TTATACT10450.013.4876324
GTCTTAA7300.013.1852291
GGTCTAG4551.1277734E-1012.8436981
GTCTTAG6700.012.8267831
GTACGAC1900.00199199912.80810550
CTATCCT7800.012.7811974
CTAGGCC7150.012.5007314
GTATAGG3852.4005203E-812.5002821
GTTCATA9100.012.4659411
GGATCGT1950.002633398912.3406886
GTACATA6800.012.1326271
GTACAAG9100.012.0881861
GTACTAA4551.551598E-912.0881861
CCCTAGG7400.012.0779751
GTTATAG5753.6379788E-1211.9567921
TCTATCC8100.011.8834113
GCCTAGA7900.011.7486841
Produced by FastQC (version 0.11.5)