FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754992
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG1300.002583522315.8665164
GTACAAA11800.015.149111
CGCACTA1400.00422251614.738008536
GTATTAG5500.013.1256981
GGTCTAG4802.0008883E-1112.891311
CTAGGAC5155.456968E-1212.6829433
GCTCTAA9000.012.6048361
GTACATA6350.012.4514481
GTATATA9950.012.4378461
GTACTAG3357.6155084E-712.3140861
GCCCTAG5601.8189894E-1212.2774381
GGTCTAA4903.9108272E-1011.9266541
GGATAGG5552.1827873E-1111.7686431
TCTATAC5853.6379788E-1211.7529743
ATTAGAC5602.5465852E-1111.6637773
CCGGACT3257.294324E-611.6354454
GTTCTAC7550.011.3830551
GTACAGG12200.011.27109051
GTATAGG5502.2191671E-1011.2505981
CTCGATT2250.00552583511.17556262
Produced by FastQC (version 0.11.5)