FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754992
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33990.1233760388414921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2951.8189894E-1218.6970561
GTCCTAG6100.018.0840381
AGTCGAC1703.9176433E-516.69061359
GTCCTAT6550.015.78902151
GTACGGG2252.067185E-615.3211981
TCTAGAC5050.014.3145973
CCCTTAG6600.014.1024661
CGTAAAC1800.00142829213.3867993
GGTTTAG5350.012.886991
GTACAAG9650.012.860281
GTCTTAG5650.012.81286051
GGTCTAA5400.012.7676651
TCTATAC5401.8189894E-1212.7493313
GGACCGA3255.282327E-712.69164856
CTATACT5700.012.6709454
GTTCTAC6400.012.3886251
GTTCTAG7550.012.3279851
GTACTAA5351.0913936E-1112.24264051
CTTAGTC4853.2196112E-1012.0658633
GTATTAG5803.6379788E-1211.8871371
Produced by FastQC (version 0.11.5)