FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812815
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG756.333519E-527.491991
GTCTTAG6800.019.7093321
GTACTAA5400.017.1824931
GTATTAG5350.016.0584051
CTAGGCG1851.0567087E-414.8607994
GCCTTAG6050.014.7684241
TAGACCG1657.4945396E-414.5793465
GTACTAG3353.426976E-914.3614881
GTACGGT2202.9193985E-514.0584051
TATACTG9000.013.7462415
CTATACT6000.013.7462394
GTCTATA4509.458745E-1112.9823281
TCTATAC5300.012.9681513
GTATAAG5650.012.7728271
GTACCGT1900.00217265112.661016
CCTATAC3852.4105248E-812.4965823
GTACAGG11000.012.496361
CCCTATA4451.0986696E-912.3559511
GTATATA10900.012.295731
TACTAGA5106.002665E-1112.1290352
Produced by FastQC (version 0.11.5)