FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812815
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32690.11621809468450645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC1056.177382E-419.641773
GTCCTAG6100.015.7938211
GGCTTAG6750.014.7826821
GCTAGAC4353.6379788E-1214.2233513
GTCTTAG6200.013.874181
GTATTAA8900.013.5312241
GTATAGG4102.9467628E-1013.42751
CTGTACC12600.013.3663654
GGTTAGG5250.013.1077971
AAATCGC2457.988818E-512.62909836
GTCCTAT5501.8189894E-1212.5119891
GCTCTAA8000.012.4728891
CTAGGAC5257.2759576E-1212.4397883
ATACCGA2509.6785894E-512.3725566
CCCTAAC5850.012.3425722
GTCTTAA7850.012.2729051
CCCCTAG6750.012.2339441
TAAGCGT2255.537116E-412.2206774
GTCTAGA7050.012.2014071
TGTACCG2551.1642133E-412.1301725
Produced by FastQC (version 0.11.5)