FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748385
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAA4350.020.5432131
GTATTAG5300.018.8064331
GCGTAAT950.00800907818.0896721
GTACATA6600.016.1436611
GTCCTAG5750.016.1391331
CTAGACT5050.014.9732344
TAGACCG1657.4855436E-414.5816375
CGTACTG1951.6512885E-414.10092455
GTACTAA4950.013.8870211
GCCCTAG5500.013.7481511
GTATTAA8800.013.2794651
CTATACT7100.013.0704264
GTACGCC1650.00988014412.96248660
TCTATAC6100.012.9593233
CTAGGGC4852.3646862E-1112.7560173
TCTGTAC11600.012.44444753
GTATAGG5257.2759576E-1212.4388041
CTAGTAC3601.3534736E-712.4115253
GTCTTAG6400.012.3518541
CGAGCGT1950.002640434812.33628816
Produced by FastQC (version 0.11.5)