FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748385
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32120.11686863376128163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG1004.446282E-420.6237354
TATGCGT1450.00534666314.2232644
GTACTAG2709.063151E-714.014991
CGTATAC1500.00669187413.7496573
GTCCTAG6400.013.4376681
TAGACGG1850.0017750813.0059595
CTACACT6950.012.8589224
ACTAACC5900.012.8174773
GTCCTAT6200.012.7614511
GCCCTAA5950.012.7194861
GTATAGG3801.9801519E-812.6738431
GTACATA6850.012.5549011
CTATACT7250.012.3268294
CAGTACT10050.012.3126764
GTTCTAG7300.012.2522081
CCTGTAC11000.012.1871953
TAGACCC4257.3014235E-912.1316085
TCTATAC6850.012.0434943
GGTCTAG6000.012.0401511
GTATTAG5552.0008883E-1111.7767241
Produced by FastQC (version 0.11.5)