FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11755220
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA83800.028.8465145
GTACTAG24450.020.3079621
CCTGTCG30800.018.83639145
GTACTAA32150.018.6766571
GTATTAG38250.016.7547631
GTCCTAG47650.016.4786931
GTGCTAG46700.015.5775971
GTATATA70500.013.9221581
GTATAGG30750.013.8941951
GTTCTAA56850.013.71041
GTCTTAG42600.013.6885841
TATACTG56100.013.6844255
GGTCTAG40100.013.5341271
CTATACT42250.013.2520434
GTTCTAG55300.013.1550411
GTCCTAA42550.013.0262211
GTATTAA60600.012.8619811
TGTACTG78750.012.7537085
GTGCTAA46350.012.5810941
CTAGTAC22050.012.5652283
Produced by FastQC (version 0.11.5)