FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11861226
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA87950.028.10796145
CGACGAA14151.5807018E-920.35414145
TGACGCG11654.365128E-516.48132145
GTCCTAG48650.016.2749421
CCTGTCG32300.015.604314145
GCCGTAT21850.015.378164145
GTACTAA38300.014.9388841
GTGCTAG48900.014.8916711
GTATTAA60650.014.3889081
TCTAGAC45500.014.2241433
GTCTTAG41300.014.1336081
GTATTAG40600.014.0925911
CTAGACC37450.014.0413554
CCGTATA10303.9494073E-513.981119145
GTTCTAG54700.013.9465991
GTTCTAA59700.013.94022751
GGTCTAG37050.013.8830051
GTATATA75500.013.7786691
GTATAGG30750.013.5322211
GTCTTAA55700.013.3849331
Produced by FastQC (version 0.11.5)