FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909652
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA89950.041.707855145
TAGCGCG9351.2956407E-617.664783145
GGGGGGG705050.017.201452145
GTACTAG22450.016.7379361
GTATTAG42900.016.4402121
GTACTAA37300.015.8087021
GTTCTAA57600.014.4525631
GTCCTAG46000.014.0755411
GTGCTAA46650.013.7554941
GTATTAA61950.013.4377351
GGTCTAA37150.013.3827241
GTCTTAA55300.013.0674161
GTATATA72300.012.9533351
GTCCTAT39450.012.895571
GTTCTAG58000.012.7581241
ACTAGCG37050.012.736897145
GTGCTAG48850.012.3076081
GTCTTAG41350.012.3030281
TGTACTG76400.012.2582985
GTATATG58000.012.1600881
Produced by FastQC (version 0.11.5)