FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909652
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA86000.027.72366145
CCTGTCG35050.019.531591145
GCCGTAA14706.545088E-817.664566145
CGACGAA16401.2121745E-817.272892145
GTACTAG23250.016.9426141
GTATTAG39550.016.6975521
GTCCTAG48350.016.0547221
CGCCGTA24450.015.447874145
GCGTAAA9500.00391048314.909233145
GTTCTAG54150.014.6560351
CTAGACG10250.014.1259464
GTGCTAG44700.014.1258121
GTCTTAG45650.013.8318451
GTCCTAA42400.013.7990731
GTATTAA63200.013.748921
CGTATAA15553.8193342E-513.662802145
GTCTTAA54700.013.6614511
GTTCTAA59300.013.4808981
GCTCTAG51150.013.47707751
CCGTATA10600.0080855413.362047145
Produced by FastQC (version 0.11.5)