FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11845768
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA94400.041.256897145
CGCGTAT7901.7826096E-929.878412145
TAGCGCG10651.09139364E-1019.946999145
CTAGCGG11151.6258964E-619.052513145
GTACTAG24550.018.8470671
GTCCTAG46250.016.8821331
GGGGGGG661250.016.670008145
GTCGCTT23601.8189894E-1116.002676145
GTATTAG43650.015.7676871
GTTCTAG55650.015.3816121
GTACTAA36700.014.9714141
GTATTAA60200.014.6033471
GTACTAT36100.013.4579031
GTACAAG68500.013.4249241
GTATATA72800.013.3469851
GTGCTAG49100.013.3107411
GTCTTAG44800.013.2973331
GTACAGG86050.012.7705041
GTATAGA46050.012.6851921
GTACATA51550.012.5659311
Produced by FastQC (version 0.11.5)