FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11845768
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA92050.027.496105145
CGCGTAT6152.0795631E-423.077423145
CGACGAA14602.7976057E-919.44194145
CCTGTCG37450.018.948753145
GTACTAA37750.017.8004821
GTCCTAA41350.017.231391
GTCCTAG48000.016.5336991
GTATTAG39600.016.2374741
GTACTAG23700.015.398661
GTGCTAG45150.015.2679221
GTCGCCG37850.014.9988145
GGTCTAG35750.014.9074211
TCGCCGT30850.014.568325145
GTTCTAG55300.014.4558841
GCTCTAG51900.014.2867471
GTCTAGA46050.014.2147591
GTCTTAG44550.013.9131921
GTCTTAA55050.013.7849351
GTATTAA61350.013.6912831
GCCCTAG45050.013.50161
Produced by FastQC (version 0.11.5)