FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8364180
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA55100.021.02276145
GTACTAG16750.018.501791
GTCCTAG33250.017.1788671
TCTAGAC29750.016.3403513
GTATTAG29150.015.8428521
CTAGACT27550.015.6601214
GGGGGGG554150.015.398734145
GTACTAA25750.014.8669431
GTATAGG22300.014.7145591
GTCCTAT29850.014.4534781
GTTCTAA39850.014.3336851
GTCCTAA29700.014.3218771
GTATTAA45500.014.0228481
GTTCTAG36050.013.6533141
TGTACCG12650.012.9699825
GTGCTAG33100.012.8507471
GTACAGG61350.012.7771351
GTCTTAT38200.012.7257961
CTATACT32300.012.6046454
GTGTAAC28850.012.42694951
Produced by FastQC (version 0.11.5)