FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8364180
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG27900.020.684143145
GTATTAG29750.017.8063141
GTCCTAG31650.017.5069051
GTTCTAG37250.016.5096111
GTCCTAT31150.015.6377311
GTTCTAA39500.015.106741
CTGTCTA54200.014.9063225145
GTACTAA27400.014.6667911
GTCCTAA27950.014.596031
GTCTACG12350.014.2979171
GTCTTAG29900.014.2550461
GTACATA36000.014.0383911
GTATAGG23900.014.0122071
GTATATA49350.013.5721061
CTATACT31400.013.5580824
GGTCTAG28350.013.530981
TGACGCG11402.3903453E-413.4991255145
GCTCTAG40650.013.181471
CCCCTAG36700.013.1069841
GTATTAA43000.013.0275011
Produced by FastQC (version 0.11.5)