FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8139263
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA63750.030.263124145
GTACTAG16350.020.5099961
GGGGGGG469650.016.99542145
GGTCTAA26750.016.6387121
GTCCTAG33300.015.5630721
GTTCTAG39000.015.4771591
CGTCTTA10009.059596E-515.131562145
GTGCTAG33450.014.7641861
GTCTTAG31100.014.70352651
GTCCTAA31500.014.3232571
GTATTAA45650.014.2910341
GTATTAG26350.014.1146411
GCTCTAG41050.014.1101321
GTACTAA25600.014.0518241
TCTAGGC36500.013.865493
GTATAGG19450.013.792841
GTATATG36800.013.7515261
GGTCTAG24900.013.7122691
TATACTG41100.013.6490155
CTATACT29850.013.48185354
Produced by FastQC (version 0.11.5)