FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8102645
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA63300.026.987303145
CGCGTAT7351.5849064E-417.87856145
GGTCTAG26000.017.34271
GTATTAG27300.017.1864571
GTCCTAG32900.016.854031
TACGCGA3055.680092E-515.9826372
GGGGGGG452300.015.730206145
GTACTAG17100.015.32253551
GTATTAA42800.014.9487131
GTTCTAG39400.014.6921611
GTACTAA25750.014.671441
GTACATA36150.014.6645681
GTGCTAG32250.014.5485361
GTTCTAA38700.014.1706511
GTATAGG22450.013.29958251
GTGCTAA34200.013.1845071
GTCTAAG28400.012.8733391
GCTCTAA37300.012.7421891
CTAGCCT30200.012.7113564
GTATACG7203.7252903E-912.6945431
Produced by FastQC (version 0.11.5)