FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6614249
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG14450.017.6639391
GTATTAG12300.016.825571
GGTCTAA12050.014.8846961
GTCTATA12650.014.4513691
GTACTAG7000.014.2897181
GTGCTAG15300.014.202761
GTACTAA12150.014.1944111
GTCTTAG13700.014.0990911
GCTAGCG2253.4839588E-513.7974893
GTGCATA13800.013.4970331
GTACAGG28200.013.454491
ATAGTAC11400.013.3133663
GTATAGG10900.013.2906561
GTATAGA13800.013.2470881
CTATACT14850.013.0077334
GTCTAGA14050.012.7658771
TTAGCGC2457.7471464E-512.6711644
GCCCTAG12650.012.5426981
CTAGACT12550.012.3682664
GTCTTAA16750.012.3554941
Produced by FastQC (version 0.11.5)