FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6614249
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7150.017.4118081
GTACTAA11300.016.525811
GTTCTAG17650.015.6744621
GTATTAA19900.015.118671
GTACTAT11450.015.1012161
GTCCTAG15500.014.9482551
GTATTAG12200.014.7397781
GTCCTAA13400.014.4520881
GGTCTAG12100.014.2899931
GTATAGG9450.014.2718481
CTAGACG3751.0622898E-913.8060284
GTGCTAG16250.013.6199031
GTGTTAG17200.013.4708131
GTCTTAG13900.013.4346511
GTCTTAA18050.013.219631
TCTAGAC12750.013.0017783
GCCCTAG14750.012.8949031
GTATAAG12600.012.6250961
CTATACT14850.012.5509354
GTTCTAA18800.012.5083061
Produced by FastQC (version 0.11.5)