FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6586247
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10350.019.6623521
GTATTAA18200.016.2986181
GTATAGG10400.015.5879241
GTTCTAG18350.015.225541
GTCCTAG14750.014.7323591
GGTCTAA13550.014.5097291
GTACATA16900.013.4704151
CTATACT16600.013.0910964
GCCTTAG15050.013.0635761
TATACTG18400.012.935255
GTGCTAG14350.012.7393651
TCTGTAC29650.012.6801833
GTCTAGA14250.012.5867111
GTATAAG11850.012.516241
GTACAGA32300.012.494161
GTACAGG29150.012.4243751
GTACTAA11750.012.3292071
GTACTAG7650.012.1737981
GTCCTAA13900.012.1592011
ATAGTAC12100.011.9725783
Produced by FastQC (version 0.11.5)