FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832578
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG16900.016.3242051
GTCCTAA15150.015.7059881
GTCCTAG16800.015.3950381
GTATTAG13050.014.5338591
GTACTAG8250.014.2119521
GTTCTAG16950.014.0380941
CTAGTAC6850.013.5935763
GTATAGG10750.013.4731641
GTATTAA19650.013.16216851
GTCTAGA13700.012.8374391
GTACAGG29600.012.8153281
GTACTAA11400.012.7049571
TCTGTAC27800.012.6536533
GTCCTAT14250.012.5839571
GTATAGA13100.012.3724391
GTTCTAA19100.012.2773411
GTCTTAA17500.012.2175011
TATACTG20250.012.09196955
GTCTATA10350.011.9947421
GTGCTAA16850.011.8701691
Produced by FastQC (version 0.11.5)