FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832578
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG15750.017.7492351
GTACTAG7550.016.9133631
GTATTAG12600.016.7083851
GTATAGG8400.016.4344791
GGTCTAA13150.015.2222011
GGTCTAG12300.014.3100211
GTGCTAG15350.013.9398641
GCCCTAG15900.013.6747261
GTATACG3801.2969394E-913.6233171
GTACATA17750.013.2216541
CTAGGCC17600.012.9319214
GCTCTAG19200.012.76241
GTATTAA20100.012.70605851
GTTCTAA18350.012.4131791
GTCTTAG15050.012.3831871
GTGTATA17100.012.3114431
GTTCTAG18300.012.2585041
GTGTTAG15650.012.128961
GTATATA20350.012.0411831
TTGTACT26100.012.0235494
Produced by FastQC (version 0.11.5)