FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6748977
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAG13450.014.3557731
GTCCTAA13750.014.2933171
GTCCTAG16250.014.216161
GTTCTAG18200.014.0191341
GTATTAG14450.013.83951951
TCTATAC13200.013.321823
GTACTAA13000.013.2613441
GTACTAT11950.012.6953771
CTAGACT13650.012.6301324
GTCTATA11800.012.5645611
GTGCGTA3852.303932E-812.5379971
CTATACT13300.012.4440034
GGTCTAA12050.012.3038851
GTCTAGA14600.012.2803681
GGATTAG13500.012.2593751
GTATAGG11050.012.1692321
TATACCG3701.8562787E-712.1146855
GTTCTAA19300.011.9695651
GTACAAG22100.011.8572011
GTCTTAG15150.011.8345451
Produced by FastQC (version 0.11.5)