FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6748977
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7350.016.9015851
GTATAAG12400.016.6971321
GTATTAG11950.015.304541
GTACTAA12900.014.7124591
GTCCTAG16500.014.4303691
GTATAGG11050.014.3650741
ATAGTAC12400.013.9080883
GTCTTAG14900.013.6640051
GTGCTAG15250.013.5766851
CCTAGAC12550.013.4670173
GGTCTAA12500.013.2508431
GTATTAA20250.013.1213341
TCTGTAC28650.012.7614593
CGATCGT3002.881341E-612.64530738
GCTCTAG17200.012.639341
GTCTTAA16550.012.5102381
GTATAGA14700.012.4414441
GTTCTAG16500.012.3390111
GGTCTAG11050.012.1790851
GCCTTAG14050.012.0346111
Produced by FastQC (version 0.11.5)