FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9689384
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA63550.038.92144145
GTACTAG18550.017.4601061
GTATTAG29350.016.2519321
GGGGGGG663700.015.483038145
GTTCTAG41350.015.3807081
GTATTAA46750.015.1156821
GTCCTAA31850.014.7913771
GTCCTAG39250.014.2531891
GCTCTAG45900.013.9843381
GTTCTAA44700.013.9645351
GGTCTAA29500.013.7738441
GTATAGA34850.013.6870651
GTCTAGG37100.013.49191
GTACAAG60150.013.3146991
TATACTG42800.013.2089045
GCGTTAG6752.3683242E-913.0862611
GTATATA50900.013.0734061
GTACGCG6351.4149919E-812.983221
TAGTACG4157.197725E-512.7712594
CAGTACT60650.012.6226884
Produced by FastQC (version 0.11.5)