FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9762879
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30650.018.874541
CGCCGTA15705.456968E-1218.745975145
GTCCTAG38950.017.5805031
GTACTAG17500.016.8660281
CTGTCTA62450.016.313398145
GCCTTAG34150.015.7301021
CCTGTCG29150.015.533015145
GTTCTAA45250.015.39374351
GTTCTAG44200.014.9581051
GTACTAT30100.014.5126271
TCTAGAC36350.014.4510593
CTAGACT33100.014.26514154
GTATTAA47900.014.1723931
GGTCTAG30850.014.1598081
CGAATCG8051.8189894E-1213.9306649
GTCTTAG34350.013.7481441
GCCCTAG39650.013.5481191
GTACTAA28550.013.4396531
CTATACT33000.013.4140964
GTGCTAA34950.013.3432231
Produced by FastQC (version 0.11.5)