FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9790861
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA68000.046.481415145
GTATTAG31450.017.0816971
GTACTAG20150.016.9927311
GTCCTAG39750.016.930821
GTGCTAG38250.015.7426911
GGGGGGG602150.015.380185145
GTATTAA48750.015.0161061
GTACTAA29150.014.986611
GTTCTAG44450.014.609361
CTAGACT31750.014.5032594
TCTAGAC34600.014.3322933
GTACTAT29450.013.8316521
GTCTTAG38850.013.8280411
GTGTATA44200.013.489921
GTACAGG72400.013.3725911
GTATAAG32700.013.1790881
GGTCTAG31250.013.0349491
CTATACG6802.5465852E-913.022584
GTGCTAA36350.012.9925921
GTTCTAA45550.012.9605041
Produced by FastQC (version 0.11.5)