FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9790861
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA66100.019.624878145
CGCCGTA20400.018.637995145
CCTGTCG34550.017.478176145
GTCCTAG39000.016.9902611
GTACTAG17800.016.6186851
GTATAGG24750.016.4937921
TGACGCG13751.1080137E-616.265886145
GTACTAA26950.016.0254691
CGACGAT50450.015.959592145
GTATTAG33650.015.1202871
GTCTTAG37350.015.0480311
GTCCTAT32400.014.9732291
CGACGAA13451.6175905E-514.965824145
GGTCTAG30900.014.5512991
CTATACT33450.014.499814
CCTATAC28100.014.3135453
GTGCTAG38800.014.0282271
GCCCTAG40750.013.9376731
GGTCTAA28950.013.8965491
GTCCTAA34100.013.8797691
Produced by FastQC (version 0.11.5)