FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726746
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA70850.042.827377145
GTACTAG18800.019.440631
GTATTAG32200.018.3071481
GTCCTAG38550.017.8911381
GGGGGGG458700.016.223751145
GTTCTAG46050.015.3613051
GTATATA49650.015.1973271
GTACTAA25750.014.8803321
GTCCTAA32350.014.7600311
CTATACT33350.014.1417834
GTATTAA45350.014.0385731
CTAGACC29150.013.95474
CTAACCT39950.013.428724
TCTAGAC34250.013.4248853
GTTCTAA44500.013.3794381
TCTATAC31950.013.2842853
GTACATG64400.013.2726821
GTGCTAG35300.013.1925551
GTACAGG73600.013.1353771
GTACAGA69850.013.0810261
Produced by FastQC (version 0.11.5)