FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726746
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG32350.022.634298145
CTGTCTA64450.022.033546145
GCCGTAT21700.021.472715145
GTCCTAG38900.018.0689851
GTACTAG19550.017.8254971
GTGCTAG38850.016.5718821
CTAGACT31850.016.1338234
CGCCGTT11105.650268E-515.991691145
GTTCTAG43300.015.9600531
GTACTAA29250.015.1450871
GTCCTAA34800.015.1059191
GCTCTAG45550.014.782671
GTATTAG30250.014.6444231
TCTAGAC34200.013.988993
GTTCTAA44750.013.8590231
GTCTTAG36300.013.8308451
GCTAGAC26950.013.5881523
GTCCTAT33950.013.5703541
CTAGACG9150.013.5558154
CGTATCA21300.013.542259145
Produced by FastQC (version 0.11.5)