FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740402
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA50150.045.159935145
GGGGGGG440050.017.84162145
GTATTAA35800.016.9531081
GTCCTAG33100.016.6223471
GTACTAA21000.016.2061771
GTATTAG25950.016.174951
GGTCTAG24950.015.4591981
GTATAGG20300.014.52967551
GTTCTAG32400.014.355471
CTATACC23000.014.3038044
GTCTTAG28100.013.9280821
GGTCTAA23300.013.8761031
GCTCTAG34850.013.6717811
GTCTTAA31250.013.6131891
GTACTAG13450.013.4951051
TATACTG32900.013.4477645
GTATATG35450.013.4403831
GTACATA29950.013.0677521
CTATACT27100.012.9769844
GTCCTAA28150.012.8958551
Produced by FastQC (version 0.11.5)