FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7815087
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA49700.048.694668145
GGGGGGG392800.019.189613145
GTCCTAG30150.016.7433661
GTATAGG20450.016.086991
GTCTTAG26800.015.6616321
GTACTAG14800.014.946621
GGTCTAG24650.014.4965191
CTAGACT24850.014.3809514
GTATTAG25350.014.096221
GTGCTAG28700.014.0319021
GTACATA31300.013.7723921
GTATATA36750.013.7363961
TCTAGAC29550.013.4365023
GTTCTAA34750.013.3844081
GTTCTAG33950.013.3656561
GTACTAA21400.013.2524551
GCTCTAG32100.013.0757551
GCACTAG21750.013.0391971
GTGTTAA33500.013.03725051
CTGTACT54450.012.7097044
Produced by FastQC (version 0.11.5)