FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7820226
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA54850.043.973892145
GGGGGGG410250.019.437567145
GTATTAG26400.017.429431
GTCCTAG30900.016.1780531
GCGCTAG4655.311449E-915.8815351
GTTCTAG32550.015.0089241
GTGCTAG29300.014.5410481
TACGCGA2757.9779164E-414.4600622
GTACAGG57700.014.3740481
GTACTAG15050.014.3433031
GTATTAA36600.013.9689411
TCTAGAC27850.013.8703853
GTATAAG24250.013.8210921
GTACAAG46050.013.6928991
GTACTAA23400.013.5948441
GTCCTAA25750.013.4571991
CGGGGGG58100.013.132005145
GTCTTAG27950.013.0076911
GTACTAT23650.012.9707371
CTAACCT30550.012.8306384
Produced by FastQC (version 0.11.5)