FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7775809
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA53950.036.37094145
CCTGTCG25300.017.671398145
GTCGCCG28200.015.854127145
GTTCTAG34700.015.7472061
GTATACG6600.015.5235241
GTCTCGT21058.394636E-915.339463145
GTGCTAG30800.015.1539161
CCGTACG8200.001522964315.145203145
GTCCTAG28450.015.0051641
CTTAACG8400.014.9070333
GCCGTAT18400.014.848882145
GTTCTAA33700.014.1876721
GGTCTAA22200.014.1016991
CGTGTAT14151.7097409E-414.042761145
GTACTAA20600.013.81543351
GTATTAA34250.013.6274631
GTGCTAA28450.013.6046831
CTATACT26850.013.5669884
GTACAGG54250.013.3249531
CTAGTAC13500.013.07000353
Produced by FastQC (version 0.11.5)