FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9332212
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11350.017.8426151
GTATTAG22400.017.1621781
GTTCTAA27850.014.6664211
TATACTG27550.013.9555735
GTCTTAG18950.013.9470991
GTACTAA18150.013.6162711
GTTCTAG25250.013.5938031
GGTCTAA17850.013.2682371
GTATTAA31900.013.0195821
GTATAGG16650.012.9875931
GTCCTAG21950.012.8227651
CCTCTAG36400.012.3529961
CCCCTAG23100.012.3329931
GTCTTAA27050.012.3085481
GTACTAT17850.012.306771
TAGTACT18200.012.2600114
GCTCTAG22950.012.1144771
GTACCGT7700.012.0374246
GTATATA35550.011.9724891
GTACAAG29550.011.9641571
Produced by FastQC (version 0.11.5)