FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9466757
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21400.018.4508761
GTACTAG12200.016.3230421
GTCCTAG22300.015.088781
GTATAGG16400.014.4456851
GTTCTAG25050.013.7064551
GTACTAA19900.013.2852751
GTTCTAA27700.013.2628531
ATAGTAC19550.013.1724263
GTACAGG39100.012.9962441
TATACTG26600.012.908595
GTCTTAG19800.012.832151
GTGCTAG22050.012.768451
CTATACT21750.012.4715124
GTCTTAA27050.012.4391791
GTCCTAA22050.012.30131151
GTATTAA31000.012.2940291
CTAGACT19000.012.2887184
GTGTATA27400.012.0296671
GTACTAT19250.011.950251
TTATACT33750.011.903194
Produced by FastQC (version 0.11.5)