FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9466757
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21850.017.1811751
GTACTAG11250.016.8379021
GTCCTAG21950.015.0631071
GTGCTAG23450.014.6870621
GTCTTAG22750.014.5334161
GTACTAA17800.014.3182361
GTTCTAG27200.013.9284121
GTATTAA32150.013.8193151
GGTCTAG16900.013.6541891
TCTAGAC19150.012.74877553
GTGTTAG23500.012.3108841
GTCTATA18600.012.2210591
GTACATA27300.012.1111781
GTCTAGA20250.012.0756671
GGTCTAA18300.012.0449971
GCTCTAG23900.011.9607391
GTACAGG37450.011.9555441
GTTCTAA28400.011.8846261
GTATAGA20950.011.8365821
CTAGACG5251.0004442E-1011.7806854
Produced by FastQC (version 0.11.5)