FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9461317
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12100.016.4755381
GGTCTAG17450.015.3637121
GTCCTAG23450.014.6573041
GTATTAG20750.014.5767781
GTCTTAG21350.014.4891061
GCCCTAG21950.014.2496391
GTGCTAG22150.013.9657961
GTACTAA17800.013.7099321
GTTCTAG26600.013.4384341
GGTCTAA17900.013.2493041
GTATTAA30250.013.1804291
GTCCTAA21850.012.8990981
GTTCTAA29750.012.8242791
TATACTG30650.012.5484335
GTATATA37350.012.5154151
TAGTACC15100.012.2809034
GTACAAG31600.012.073491
CTATACT24100.011.9694924
GCTCTAA27300.011.9607361
TAGGACG6350.011.8976844
Produced by FastQC (version 0.11.5)