FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9470745
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22700.016.4886461
GTCCTAG21850.016.0299841
GTCTTAG20100.015.5463941
GTTCTAG26750.014.6340791
GGTCTAG16950.014.181191
GTACTAG13350.013.8898261
GTGCTAG23100.013.8246871
GTACTAA18750.013.3692151
GTATATG30700.013.1986031
GTATTAA32600.013.1666941
GTACTAT18650.013.0726571
GTTCTAA29700.012.833671
GCTCTAG23150.012.7565071
GTATACG5501.8189894E-1212.48681451
GTACAGG39850.012.3223071
GTCCTAA20900.012.1582141
GCCCTAG20950.012.1291961
CTACACT28750.012.0639854
CTTATAC21800.011.971873
GTATAAG23250.011.815481
Produced by FastQC (version 0.11.5)