FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9470745
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11700.017.6235451
GTCCTAG21650.015.3971981
GTACTAA18250.015.2528151
GTATTAG22100.014.4616741
GTTCTAA29000.014.3388811
GTTCTAG27150.014.3033071
GCTCTAG25250.013.6102621
TCTATAC22250.013.5836573
GTATATA37800.013.1826911
TCTAGAC20100.012.9861853
GTATTAA30900.012.9011181
GTCTTAG22650.012.4415231
GTCTTAA26850.012.2872531
GCCCTAG22550.012.1918971
GTCCTAA21700.012.0359871
GGTCTAG18100.011.9616161
TCTGTAC39750.011.8371593
GTCCTAT18950.011.7877791
GTATTAT34250.011.6392571
GTACAAG30650.011.4366161
Produced by FastQC (version 0.11.5)