FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9097347
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA70750.022.372776145
GTACTAG20550.019.4182741
GTATTAG37000.016.7582531
GTCCTAG36400.016.5285131
GTCTTAG36400.016.1911961
GTATAGG30050.015.9352621
GTACTAA31600.015.3479051
CCTGTCG26200.015.285733145
GTTCTAG46550.015.1665661
CTGTACT75650.014.9890514
CTATACT40100.014.8265034
TCTAGAC36700.014.7017443
GTGCTAG38750.014.575561
GTCTTAA46050.014.531351
TATACTG54450.014.51954655
GTATATA74750.014.4547491
TATACCG8950.014.3799545
GTATTAA61050.013.9777821
CCTATAC28500.013.7685233
CTATACC33700.013.6408914
Produced by FastQC (version 0.11.5)